Journal: Journal of Cellular and Molecular Medicine

Article Title: Honokiol ameliorates angiotensin II ‐induced cardiac hypertrophy by promoting dissociation of the Nur77–LKB1 complex and activating the AMPK pathway

doi: 10.1111/jcmm.18028

Figure Lengend Snippet: HNK inhibits cardiomyocyte hypertrophy in vivo and in vitro . (A) Representative LV sections stained with haematoxylin and eosin (H&E). Scale bar: 2000 μm. (B–E) Representative LV sections stained with H&E and Sirius red (SR) to evaluate cardiomyocyte hypertrophy (B) and fibrosis (D), respectively. Scale bar: 50 or 200 μm. (C) Quantification of the cardiomyocyte cross‐sectional area in the H&E‐stained sections. (E) Quantification of collagen in the SR‐stained cardiomyocytes. (F) Levels of galectine‐3 (Gal‐3) at the end of the study, as measured using ELISA assay. ns indicates no statistical significance. (G) Representative immunoblots and quantification of ANP protein upon treatment of NRVMs with Ang II (10 −7 mol⋅L −1 ) for 24 h ( n = 3). (H,I) Representative confocal IF images of CD31 and IB4 obtained from capillaries and arteries in LV and the quantification of the positive area of ORI. (J) Representative LV sections stained with TUNEL.DAPI staining denotes nuclei; myocardial apoptosis index measured as the positive cell divided by total cell. Data have been shown as mean ± S.E.M. n = 6–9 rats per group; ** p < 0.01, as assessed using one‐way anova followed by Tukey's multiple comparison test. CTL, control; AII, angiotensin II; HNK, honokiol.

Article Snippet: The serum levels of natriuretic peptide A, natriuretic peptide B, galectin‐3 (Gal‐3) and Ang II were analysed by employing an ELISA kit (Cloud‐Clone Corp).

Techniques: In Vivo, In Vitro, Staining, Enzyme-linked Immunosorbent Assay, Western Blot, TUNEL Assay, Comparison